Coding

Part:BBa_K131027:Design

Designed by: Thane Kubik   Group: iGEM08_Calgary_Wetware   (2008-10-27)


ColE3 operon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2444
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2444
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2444
    Illegal XhoI site found at 2510
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2444
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2444
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Escherichia coli plasmid pColE3-CA38 colicin E3, immunity protein E3, immunity protein E8, and lysis protein genes, complete cds. Accession: J01574.


Source

Escherichia coli plasmid pColE3-CA38 colicin E3, immunity protein E3, immunity protein E8, and lysis protein genes, complete cds. Accession: J01574.

References